Chalgrove Brook is a chalk stream located in South Oxfordshire, England.
It is a left tributary of the River Thame. It rises in a number of natural springs at the foot of the Chiltern Hills in and near Watlington, and flows in a northwesterly direction for approximately through Cuxham, Chalgrove, and Stadhampton, before emptying into the Thame near Chiselhampton.
The brook floods occasionally, especially in and downstream of Chalgrove. To relieve this, a man-made conduit was built in Chalgrove, splitting the stream in two.
The stream is home to brown trout, mink, otter and water vole, and possibly also the endangered white-clawed crayfish. As part of the brook's conservation efforts, in 2023 a fish pass was created to circumvent Stadhampton Mill, making available of the brook that was previously impassable to fish.
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